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The European Arabidopsis Stock Centre

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NASC ID: N849601

Name: WiscDsLox245C01

ABRC stock number: CS849601

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox245C01.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G03030.1 and AT2G03040.1 (chr 2 pos 891279 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796472.

Wiscseq_DsLox245C01.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G44045 and AT3G44050.1 (chr 3 pos 15818359 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796473.

Wiscseq_DsLox245C01.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16230.1-UTR3 and AT4G16235-P355 (chr 4 pos 9187298 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796474.

Donation Date: 2004-12-01

Donated by:  University of Wisconsin, Madison Patrick Krysan
University of Wisconsin, Madison Richard Amasino
University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox245C01

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status Price (£)
Available to order at NASC £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code: